Reporter

Part:BBa_K1718001:Design

Designed by: Naomi Iverson   Group: iGEM15_CU_Boulder   (2015-09-13)


pBAD promoter with RBS-RFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1791
    Illegal AgeI site found at 1903
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

We removed the RFP promoter (from BBa_J04450) so that RFP would only be transcribed when pBAD was stimulated by arabinose.


Source

All parts from this construct come from the registry.

References